HBTU

Drive high-efficiency amide bond formation and advanced synthetic workflows with HBTU (CAS No.: 94790-37-1, CAT No.: SRCH-023). HBTU (O-Benzotriazol-1-yl-N,N,N’,N’-tetramethyluronium hexafluorophosphate) is a leading coupling reagent for solid-phase and solution-phase peptide synthesis, widely used for its reliability, low racemization rates, and high yields. Mechanistically, HBTU activates carboxylic acids to form stable O-benzotriazolyl (HOBt) esters, accelerating acylation of amines with minimal side reactions and racemization. Its uronium/guanidinium N-oxide structure provides enhanced reactivity and compatibility with Fmoc and Boc strategies, as well as diverse peptide and small-molecule coupling applications. HBTU is highly soluble in DMF, acetonitrile, and similar solvents, exhibits fast reaction kinetics, and is safe to handle with standard laboratory precautions.

Molecular formula: C₁₁H₁₆F₆N₅OP, Molecular weight: 379.24 g/mol. HBTU is supplied as a white to off-white crystalline powder with purity ≥98%. Store in a cool, dry place protected from moisture and light for optimal stability.

Product Highlights

Technical Features & Benefits

• Highly Efficient Coupling: Excellent yields with low racemization – ideal for solid and solution-phase peptide synthesis.
• Fast Kinetics: Very short reaction times and mild conditions for sensitive substrates.
• Broad Compatibility: Suitable for Fmoc/Boc strategies, cyclization, and fragment condensation in peptide assembly.
• Stable Reagent: Long shelf life, robust for automated and manual synthesis protocols.

Application Areas

• Solid-phase and solution-phase peptide synthesis (SPPS and LPPS)
• Acylation and coupling of carboxylic acids with amines and alcohols
• Synthesis of difficult, sterically hindered, or acid-sensitive peptides
• Oligonucleotide and small-molecule coupling protocols

Safety & Handling

• Precautions: Wear gloves, goggles, lab coat. Avoid direct contact, inhalation, and ingestion. Handle in a well-ventilated area.
• Storage: Keep in a sealed container, in a cool, dry, dark location under inert gas if possible, away from light and moisture.
• Hazards: Causes skin and eye irritation, may cause allergic skin reactions. Not for food, drug, or household use.
• Disposal: Dispose in accordance with local and national chemical regulations as hazardous waste.

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• Expert Manufacturing: Produced to exacting purity standards in state-of-the-art facilities for trusted, reproducible results.
• Custom Synthesis: Bulk and tailored formulations available to meet diverse research and production needs.
• Full Quality Control: Each batch supplied with COA and advanced analytical (NMR, LC-MS, HPLC) data.
• Dedicated Customer Support: Responsive communication and flexible supply solutions for R&D and production labs.

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Take your research further with SRIRAMCHEM’s premium peptide coupling reagents. Contact us for competitive pricing, samples, or bulk custom supply tailored to your requirements.