H-Val-AMC

Enhance your protease assay and peptide research with H-Val-AMC (CAS No.: 78682-66-3, CAT No.: SRCV-005), a fluorogenic substrate consisting of the amino acid valine linked to 7-amino-4-methylcoumarin (AMC). This compound is widely used as a sensitive and specific substrate for detecting protease activity, particularly in enzymatic assays for cathepsins, caspases, and other proteolytic enzymes.

Supplied as a white to off-white powder with high purity (≥98%), H-Val-AMC is soluble in aqueous buffers and organic solvents, providing convenient handling for spectrofluorometric and kinetic assays. Upon enzymatic cleavage of the peptide bond, the AMC fluorophore is released, resulting in a measurable increase in fluorescence intensity, enabling rapid and quantitative protease activity evaluation.

Product Highlights

Technical Features & Benefits

• Fluorogenic Substrate: Releases highly fluorescent 7-amino-4-methylcoumarin (AMC) upon enzymatic cleavage, enabling sensitive protease activity assays.
• High Purity: Ensures specificity and reliability in enzymatic and biochemical assays.
• Good Solubility: Compatible with both aqueous and organic solvents for versatile assay conditions.
• Stable Compound: Suitable for storage and repeated use in laboratory settings.
• Applicable in Enzymology: Ideal for detecting protease activities in physiological and pathological studies.

Application Areas

• Protease and peptidase enzymatic activity assays
• Drug discovery and inhibitor screening
• Biochemical research on proteolytic pathways
• High-throughput screening and diagnostic assays

Safety & Handling

• Handling Precautions: Use gloves, safety glasses, and lab coat. Avoid ingestion, inhalation, and skin or eye contact.
• Storage Recommendations: Store in a sealed container at -20°C, protected from moisture and light.
• Disposal: Dispose of according to local regulations and institutional protocols.

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• Specialized Biochemical Reagents: Providing high-quality substrates for protease assays and enzymology research.
• Custom Synthesis and Scale-Up: Flexible options tailored to research and industrial requirements.
• Full Analytical Support: COA, NMR, HPLC, and purity data provided with each batch.
• Customer Service Excellence: Dedicated technical and order support from inquiry to delivery.

Request a Quote or Sample Today!

Advance your enzymology and protease research with SRIRAMCHEM’s premium H-Val-AMC. Contact us for pricing, sample availability, or custom synthesis solutions.