H-Phe-AMC

Enhance your enzymology, protease research, and biochemical assays with H-Phe-AMC (CAS No.: 98516-72-4, CAT No.: SRCP-013). Also known as L-phenylalanine 7-amido-4-methylcoumarin, this compound is a widely used fluorogenic substrate for serine and cysteine proteases. Upon enzymatic cleavage, it releases the highly fluorescent AMC group, which allows for sensitive quantification of protease activity in real-time kinetic assays, drug screening, and protein characterization workflows.

The molecular formula is C₁₉H₁₈N₂O₃, and the molecular weight is 322.36 g/mol. H-Phe-AMC is typically supplied as a white to off-white crystalline powder, highly soluble in DMSO and other polar organic solvents. It should be stored at -20 °C, protected from light and moisture, to preserve stability and fluorescence performance.

Product Highlights

Technical Features & Benefits

• Fluorogenic Substrate: Releases a highly fluorescent AMC group upon enzymatic cleavage, allowing real-time and sensitive assay readouts.
• Wide Research Utility: Applicable in enzyme activity measurement, inhibitor screening, and protein functional studies.
• Reliable and Reproducible: Manufactured with guaranteed purity (≥98%) for consistent and robust experimental outcomes.
• Easy to Use: Soluble in DMSO and other solvents for convenient assay setup and stock solution preparation.
• Stable Under Proper Storage: Maintains assay sensitivity when stored under recommended conditions, minimizing background fluorescence.

Application Areas

• Enzymatic activity assays for serine and cysteine proteases
• High-throughput inhibitor screening and drug discovery programs
• Real-time kinetic measurements in biochemical and clinical research
• Detection and quantification of protease activity in biological samples
• Fundamental protein degradation and cellular process studies

Safety & Handling

• Use personal protective equipment: gloves, lab coat, and safety glasses
• Avoid inhalation, ingestion, and contact with eyes or skin
• Handle under low-light conditions to preserve fluorescence activity
• Store in sealed containers at -20 °C with protection from moisture and air
• Dispose of any waste according to institutional and environmental regulations

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• Longstanding expertise in high-purity enzyme substrates and biochemicals since 2013
• Full lot-specific documentation, including COA and spectral data
• Consistent availability with support for bulk research and OEM needs
• Expert customer and technical support for all research phases

Request a Quote or Sample Today!
Enhance your enzyme and protease research with SRIRAMCHEM’s H-Phe-AMC. Contact our team for fast pricing, samples, or for custom packing options for your screening program.