H-His-AMC

Elevate your enzymatic assay and peptide research with H-His-AMC (CAS No.: 191723-64-5, CAT No.: SRCH-024). This fluorescently labeled dipeptide combines histidine with 7-amino-4-methylcoumarin (AMC), a widely used fluorogenic moiety for studying protease and peptidase activity. The histidine residue provides biochemical specificity through its imidazole side chain, while the AMC label enables sensitive detection of peptide bond cleavage by emitting fluorescence upon enzymatic hydrolysis. H-His-AMC is soluble in aqueous and organic solvents, making it ideal for high-throughput screening, kinetic studies, and biomolecular interaction assays. This compound is valuable as a substrate for enzyme activity monitoring and drug discovery applications.

Molecular formula: C₁₅H₁₇N₅O₃, Molecular weight: approximately 311.33 g/mol. It is typically supplied as a white to off-white powder with ≥98% purity, ensuring reliability and reproducibility in experimental setups.

Product Highlights

Technical Features & Benefits

• Fluorogenic Peptide: Incorporates AMC fluorophore enabling sensitive detection of proteolytic activity.
• Histidine Residue: Provides metal binding and catalytic properties relevant to enzymatic function studies.
• High Purity: Ensures consistent performance in enzymology and fluorescence assays.
• Versatile Solubility: Compatible with aqueous and organic solvents for varied assay conditions.
• Wide Applications: Suitable for protease screening, kinetic studies, inhibitor evaluations, and drug discovery.

Application Areas

• Protease and peptidase activity assays.
• High-throughput screening of enzyme inhibitors.
• Studies on enzyme kinetics and substrate specificity.
• Fluorescence-based biochemical and diagnostic assays.
• Drug discovery and pharmaceutical research.

Safety & Handling

• Precautions: Use PPE including gloves, goggles, and lab coat. Avoid inhalation, ingestion, and contact with skin and eyes.
• Storage: Store tightly sealed at -20°C, protected from light and moisture to maintain stability.
• Disposal: Dispose of according to local and national chemical waste regulations.

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• Expert Manufacturing: Produced with high purity and stringent quality control for reliable research outcomes.
• Custom Synthesis: Scalable production options tailored to your research demands.
• Comprehensive Documentation: Each batch is supplied with Certificate of Analysis (COA) and advanced analytical data including NMR and LC-MS.
• Responsive Support: Dedicated customer service to assist with your scientific needs from inquiry to delivery.

Request a Quote or Sample Today!
Advance your enzymology research and assay development with SRIRAMCHEM’s quality-assured fluorescent peptides. Contact us for pricing, samples, or custom synthesis requests.