H-Ala-AMC

Optimize your fluorogenic enzyme assays and peptide research with H-Ala-AMC (CAS No.: 77471-41-1, CAT No.: SRCA-030), a high-purity fluorogenic substrate for aminopeptidase M and related proteases. This compound, also known as L-Alanine-7-amido-4-methylcoumarin, is characterized by a terminal alanine residue linked to the fluorophore 7-amino-4-methylcoumarin (AMC).

With molecular formula C₁₃H₁₄N₂O₃ and molecular weight 246.26 g/mol, H-Ala-AMC appears as a white crystalline powder, valued for its high purity (≥98%) and DMSO solubility. Upon enzymatic cleavage, it liberates AMC, yielding a highly fluorescent product instrumental in quantitative protease and peptide hydrolase activity studies. Ideal for biochemical assay development, kinetic studies, and drug screening applications.

Product Highlights

Technical Features & Benefits

• Fluorogenic Substrate: Upon enzymatic cleavage, liberates highly fluorescent AMC for sensitive detection of enzyme activity.

• High Purity and Quality: Manufactured to ≥98% purity for reproducible biochemical and analytical assays.

• Solubility: Dissolves readily in DMSO, simplifying assay preparation and stock solution handling.

• Stability: Stable when stored at -20°C, maintaining activity for at least 1 month (longer at -80°C).

• Broad Applicability: Essential for kinetic enzyme assays, inhibitor screening, and drug discovery research.

Application Areas

• Fluorometric assays for aminopeptidase and peptidase activity detection

• High-sensitivity kinetic and screening studies in enzymology

• Drug discovery and inhibitor characterization involving peptidases

• Biochemical studies on protein turnover and metabolism

Safety & Handling

• Handling Precautions: Use personal protective equipment (gloves, goggles, lab coat). Avoid inhalation, ingestion, and contact with skin or eyes.

• Storage Recommendations: Store tightly closed at -20°C in a dry, dark location; avoid repeated freeze/thaw cycles.

• Disposal: Dispose according to local, regional, and national environmental regulations.

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• Expert Manufacturing: Advanced synthesis and stringent quality controls ensure batch-to-batch reliability and high purity since 2013.
• Custom Synthesis: Custom sizes and derivatives for fluorogenic substrates and peptide research, from mg to multi-gram quantities.
• Uncompromising Quality: Supplied with Certificate of Analysis (COA) and detailed analytical documentation for your assurance.
• Customer-Centric Approach: Responsive technical support and personalized solutions from inquiry to delivery and beyond.

Request a Quote or Sample Today!
Accelerate your protease assays and biochemical research with SRIRAMCHEM’s high-quality H-Ala-AMC. Contact our expert team now for competitive pricing, samples, or custom synthesis inquiries.