Gly-7-Amino-4-Methylcoumarin TFA Salt

Advance your enzymology, peptide synthesis, and biochemical assay development with Gly-7-Amino-4-Methylcoumarin TFA Salt (CAT No.: SRCG-010). This compound is a fluorogenic substrate consisting of glycine linked to 7-amino-4-methylcoumarin (AMC) conjugated to trifluoroacetate (TFA) as a counterion. It is widely used in enzyme assays for detecting proteolytic activity by releasing fluorescent AMC upon enzymatic cleavage.

Gly-7-Amino-4-Methylcoumarin TFA Salt is typically supplied as a white to off-white crystalline powder, soluble in aqueous and organic solvent mixtures including dimethyl sulfoxide (DMSO) and dimethylformamide (DMF), facilitating flexible use in enzymatic and biochemical assay protocols.

Product Highlights

Technical Features & Benefits

• Fluorogenic Substrate: Releases fluorescent 7-amino-4-methylcoumarin upon cleavage, providing sensitive and real-time monitoring of protease activity.
• TFA Salt Form: Enhances compound stability, solubility, and ease of handling.
• High Purity: Ensures reproducible assay results and minimal background fluorescence.
• Versatile Utility: Suitable for use in various biochemical assays including protease screening, enzyme kinetics, and inhibitor testing.
• Compatibility: Soluble and stable in common assay buffers and organic solvents.

Application Areas

• Fluorometric assays for protease and peptidase activity analysis.
• Screening and kinetic characterization of enzyme inhibitors.
• Biochemical studies involving peptide substrate specificity and enzyme kinetics.
• Use in drug discovery workflows targeting protease enzymes.
• Development of high-throughput screening (HTS) assays for diagnostic and research purposes.

Safety & Handling

• Wear appropriate personal protective equipment including gloves, lab coat, and eye protection.
• Avoid inhalation, ingestion, and skin or eye contact.
• Store tightly sealed in a cool, dry place protected from light and moisture.
• Dispose of chemical waste according to local, regional, and national regulations.

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• Quality-controlled manufacturing with ≥98% purity and batch consistency since 2013.
• Comprehensive analytical documentation including COA, NMR, HPLC, and LC-MS provided.
• Custom synthesis and scale-up services tailored to your research needs.
• Responsive technical support and dedicated customer service for your project success.

Request a Quote or Sample Today!
Enhance your enzymology assays and peptide research with SRIRAMCHEM’s Gly-7-Amino-4-Methylcoumarin TFA Salt. Contact our expert team for pricing, availability, or custom synthesis inquiries.