Fmoc-His(Boc)-OH

Advance your peptide synthesis and medicinal chemistry research with Fmoc-His(Boc)-OH (CAS No.: 81379-52-4, CAT No.: SRCF-039). This high-purity amino acid derivative features L-histidine protected at the α-amino terminus with a 9-fluorenylmethoxycarbonyl (Fmoc) group and the imidazole side chain protected with a tert-butoxycarbonyl (Boc) group. It is an important building block for solid-phase peptide synthesis (SPPS), providing selective side-chain protection to prevent undesired reactions during peptide assembly and ensuring high sequence fidelity.

The compound has a molecular formula of C₂₁H₂₄N₄O₄ and a molecular weight of approximately 392.44 g/mol. Fmoc-His(Boc)-OH typically appears as a white to off-white crystalline powder, soluble in commonly used peptide synthesis solvents such as dimethylformamide (DMF), dimethyl sulfoxide (DMSO), and N-methyl-2-pyrrolidone (NMP), enabling efficient coupling and seamless integration into both automated and manual peptide synthesis workflows.

Product Highlights

Technical Features & Benefits

• Dual Protection: Fmoc protects the α-amino group with base-labile protection while the Boc group selectively protects the histidine imidazole side chain to prevent side reactions.
• High Purity: Manufactured under stringent quality control ensuring reproducible coupling efficiency and peptide synthesis consistency.
• Excellent Solubility: Suitable for use in automated and manual peptide synthesis workflows due to compatibility with common peptide solvents.
• Stable and Reliable: Provided as a crystalline powder ensuring ease of handling, accurate weighing, and long shelf life.
• Versatile Use: Critical intermediate for synthesizing histidine-containing peptides, bioactive sequences, and conjugates with controlled side-chain protection.

Application Areas

• Solid-phase and solution-phase peptide synthesis (SPPS) involving histidine residues.
• Medicinal chemistry for preparation of peptides and proteins with selective Boc-protected histidine side chains.
• Synthesis of histidine-containing enzyme substrates, inhibitors, and bioactive peptides.
• Development of peptide-based therapeutics, probes, and molecular biology reagents.
• Structure-activity relationship (SAR) studies and protein engineering involving histidine modifications.

Safety & Handling

• Use appropriate personal protective equipment including gloves, safety goggles, and lab coat.
• Avoid inhalation, ingestion, and contact with skin or eyes.
• Store sealed in a cool, dry, well-ventilated place protected from moisture and direct light.
• Dispose of chemical waste following local, regional, and national regulations.

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• Expert manufacturing facilities with stringent quality assurance delivering ≥98% purity and batch-to-batch consistency since 2013.
• Comprehensive analytical documentation including Certificate of Analysis (COA), NMR, HPLC, and LC-MS accompanies every batch.
• Custom synthesis and scale-up capabilities tailored to your peptide synthesis and pharmaceutical development needs.
• Responsive technical support and dedicated customer service ensuring success throughout your research lifecycle.

Request a Quote or Sample Today!
Accelerate your peptide synthesis and medicinal chemistry research with SRIRAMCHEM’s premium Fmoc-His(Boc)-OH. Contact our expert team now for pricing, sample availability, or custom synthesis inquiries.