H-Pro-AMC

Accelerate your enzymology, protease assays, and biochemical research with H-Pro-AMC (CAS No.: 96643-94-6, CAT No.: SRCP-017). Also known as L-proline 7-amido-4-methylcoumarin, this compound is a fluorogenic substrate utilized principally for the sensitive detection and quantification of prolyl-specific protease activities. Upon enzymatic cleavage, it liberates the highly fluorescent AMC (7-amino-4-methylcoumarin) moiety, facilitating real-time monitoring of protease activity in inhibitor screening, kinetic measurements, and protein characterization studies.

The molecular formula is C₁₅H₁₆N₂O₃, and the molecular weight is 272.30 g/mol. H-Pro-AMC is supplied as a white to off-white crystalline powder, highly soluble in DMSO and polar organic solvents. For best performance and stability, it should be stored at -20 °C, protected from light and moisture.

Product Highlights

Technical Features & Benefits

• Fluorogenic Peptidic Substrate: Releases a strongly fluorescent AMC group allowing ultrasensitive quantification of protease activity.
• Prolyl Protease Specificity: Ideal for detecting prolyl endopeptidase and related enzyme actions in biological and clinical samples.
• High Purity: Guaranteed ≥99% purity ensures accurate, reliable assay results for research and drug discovery.
• Convenient Solubility: Easily dissolved for rapid assay preparation and kinetic studies.
• Documentation & Support: Delivered with certificate of analysis and technical documentation for seamless lab integration.

Application Areas

• Protease and peptidase activity assays, especially for prolyl endopeptidase
• High-throughput inhibitor screening in pharmaceutical R&D
• Real-time kinetic and mechanistic studies of enzyme function
• Characterization of enzyme substrates and drug candidates
• Biochemical diagnostics and research in protein degradation

Safety & Handling

• Use PPE: gloves, lab coat, and protective eyewear
• Avoid inhalation, ingestion, and contact with skin or eyes
• Store tightly sealed, protected from humidity, at -20 °C
• Dispose of waste in accordance with institutional and governmental guidelines

Why Choose SRIRAMCHEM Laboratories Pvt. Ltd.?

• High-purity fluorogenic substrates and biochemical tools since 2013
• Comprehensive documentation and technical support
• Options for custom synthesis, bulk supply, and guaranteed traceability
• Dedicated customer support for high-quality research outcomes

Request a Quote or Sample Today!
Boost your enzyme assays and proteomics research with SRIRAMCHEM’s H-Pro-AMC. Contact us for pricing, sample requests, and technical assistance.